重组人OX40蛋白（Recombinant Human OX40 Protein），GMP级

产品货号：GMP-TL725

产品描述（Description）

Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40 / TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4 / OX40L / GP34 and can interacts with TRAF2, TRAF3 and TRAF5.

分子别名（Synonym）

TNFRSF4、CD134、OX40L receptor、ACT35、TXGP1L

表达宿主（Expression host）

HEK293细胞

来源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列编码人OX40（NCBI Reference Sequence:NP_003318.1）表达带有His标签在C末端

分子量（Molecular Weight）

重组人OX40蛋白包含189个氨基酸，预测理论分子量为21.8 kD

纯度（Purity）

≥95%采用SDS-PAGE凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定人OX40(His 标签)1ug/mL (100uL/孔)能结合人OX40 Ligand(Fc 标签)的线性范围是1-10ng/mL

稳定性（Stability）

冻干的样本的可以在4℃保存24个月，溶解后的液体可以于-20℃保存6-12个月，并且避免反复冻融

组成（Component）

含有6%甘露醇的无菌PBS（PH 7.4）的冻干粉

参考文献

1、1. Fc Engineering Approaches to Enhance the Agonism and Effector Functions of an Anti-OX40 AntibodyDi Zhang, Monica V. Goldberg, Mark L. ChiuJ Biol Chem. 2016 Dec 30; 291(53): 27134–27146. Published online 2016 Nov 17. doi: 10.1074/jbc.M116.757773 

2、Small-molecule modulators of the OX40–OX40 ligand co-stimulatory protein–protein interaction Yun Song, Emilio Margolles-Clark, Allison Bayer, Peter Buchwald Br J Pharmacol. 2014 Nov; 171(21): 4955–4969. Published online 2014 Oct 24. doi: 10.1111/bph.12819 

3、PD-1 co-inhibitory and OX40 co-stimulatory crosstalk regulates helper T cell differentiation and anti-Plasmodium humoral immunity Ryan A. Zander, Nyamekye Obeng-Adjei, Jenna J. Guthmiller, Divine I. Kulu, Jun Li, Aissata Ongoiba, Boubacar Traore, Peter D. Crompton, Noah S. Butler Cell Host Microbe. Author manuscript; available in PMC 2016 May 13.Published in final edited form as: Cell Host Microbe. 2015 May 13; 17(5): 628–641. Published online 2015 Apr 16. doi: 10.1016/j.chom.2015.03.007

   注意：For research and manufacturing use.